SigmaPlot Enzyme Kinetics Module |
Enzyme Kinetic Analysis Doesn't Get Any Easier
The Enzyme Kinetics
Module is an add-on to SigmaPlot
that provides the curve fitting
and graphing capabilities you need
to analyze and present your enzyme
kinetics data - quickly and easily.
The module follows SigmaPlot's
tradition of award-winning interface,
ease-of-use, and intelligent wizards
to guide you through your entire
analysis. Just import or enter
your data, select the type of study
and the equation you would like
to fit, as well as the interactive
graphs to display your results.
Using SigmaPlot's powerful non-linear
curve-fitter, the module fits the
selected equations to your data,
as well as provides interactive
graphs you need to see and study
the kinetics mechanism. What's
more, you get a detailed report
complete with all statistical parameters
for each model you fit so that
you can easily compare the different
models to identify the best one
for your data. It's that easy!
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Select From a Wide Range of Built-In Models
Select from multiple
equation groups to analyze your
data. The module provides over
40 different built-in equations
to characterize different types
of reaction mechanisms. It automatically
estimates the initial parameters
for the selected model(s), and
uses the Marquardt-Levenberg algorithm
to determine the parameter values.
You get a detailed statistical
report as well as a data report
that includes the Km, Ki and Vmax
values and their standard errors.
You can simultaneously fit and
compare multiple models and graphs
from library of built-in equations.
You can also enter your own custom
equations and save them for future
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Simplified
Data Management to Organize Your Data and
Results
Fast analysis starts with the module's
Data Entry Wizard. The Data Entry Wizard
provides streamlined data entry into
the SigmaPlot worksheet. It automatically
determines the data column assignments
based on the study type you select
and makes setting up your data into
the worksheet a breeze. Cut and paste
data from any application or enter
your data manually. The module allows
you to select the units of your velocity
or other dependent variable values
from a drop down list. These units
are carried to the worksheet and also
into the final graphs. Replicate velocities
may be entered, and for inhibition
studies, you can choose to vary either
the substrate or the inhibitor concentrations
for each series. Select from lists
of unit values for substrate and velocity
or enter your own; the units are carried
through to the reports and graphs.
And once you run your analysis, you
can organize your reports, graphs and
equations in the SigmaPlot Notebook.
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Easily Determine the Best-Fit Inhibition Model for Your Data
To compare the
different inhibition models, the
Enzyme Kinetics Module provides
a detailed report with complete
statistical analysis for each model,
as well as provides information
on comparing the different models.
Several goodness of fit criteria
including Akaike's AIC are provided
to determine the best model. Statistical
results include parameter value
confidence intervals, a residual
runs test and outlier detection.
The data report includes replicate
mean velocities and their standard
errors, the predicted and residual
values from the curve fit and an
indication of data outliers. The
statistical report generated for
each model includes the parameter
values with their standard errors
and 95% confidence intervals, Akaike,
R2, sum of squares, standard error
of the regression and the significance
level for the runs test. The selected
models can be automatically ranked
by goodness of fit. [top]
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See Results Clearly With Interactive Graphs
You can create any one or all
of the built-in graphs provided
for each study. The module automatically
generates interactive graphs you
choose to display your results.
You can modify the graphs as desired
to present your results exactly
the way you want to using SigmaPlot's
ability to customize your graphs.
Many of the graph algorithms automatically
determine where fit lines intersect
the axes and present the graphs
in this manner. To help determine
the type of inhibition, the curve
fit lines are designed to allow
you to extend the graph axes to
show fit line intersection. [top]
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Publish Your Work Anywhere
The Enzyme Kinetics
Module runs seamlessly with SigmaPlot.
You can easily create custom reports
of your results including tables
and graphs, incorporate your graphs
into presentations or export your
graphs into a wide range of graphic
file formats to send them for publishing
in journals. You can also create
an HTML output of your graph and
report and post it on the internet.
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Plus, the Power of SigmaPlot
In addition to
specialized analysis and graphs
the Enzyme Kinetics Module provides,
you have access to all of SigmaPlot's
analytical, graphing and presentation
capabilities! You can get started
and publish your results faster
than you ever imagined. And, SigmaPlot
provides the flexibility you need
to customize your graph and present
your results exactly the way you
want to. In fact, SigmaPlot's graphing
flexibility has inspired the readers
of Scientific Computing and Instrumentation
to award SigmaPlot their prestigious
Reader's Choice Award eight years
running! [top]
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